高效液相色谱法测定野菊花中木犀草苷和蒙花苷含量

Determination the Contents of Luteolin-7-o-glucoside and Buddleoside in Chrysanthemum Indicum L.by RP-HPLC

目的:建立RP-HPLC法对野菊花中木犀草苷和蒙花苷同时定量方法,考察不同产地野菊花中木犀草苷、蒙花苷的含量。方法:用梯度洗脱。色谱柱为:Phenomenex C_(18)(250 mm×4.6 mm,4μm),柱温:35℃,流动相分别为甲醇和-1%冰醋酸溶液,流速为1.0 ml·min^(-1),检测波长为350 nm。结果:木犀草苷、蒙花苷浓度与峰面积呈良好线性关系,线性范围分别是:木犀草苷0.012～O.554μg,r=0.9992,加样回收率为97.5%,RSD为2.8%(n=6);蒙花苷0.005～0.197μg,r=0.9998,加样回收率为97.1%,RSD为1.7%(n=6)。结论:该方法快速,准确,适用于测定野菊花中樨草苷和蒙花苷的含量。

Objective： To establish a HPLC method for determinations the contents of Luteolin-7-o-glucoside and Buddleoside in chrysanthemum Indicum L. Method： The Phenomenex C18 column（250 mm×4.6 mm,4 μm） was used, the methanol and -0.1% glacial acetic acid slution as the mobile phase, the flow rate was 1.0 ml·min^-1, The Linear gradient elution was applied. The detection wavelength was 350 nm. Result： The linear ranges of luteolin-7-o-glucoside and buddleoside were at 0.012 - 0.554 μg （ r = 0.999 2） and 0.005 -0. 197 μg（r =0.999 8） , respectively, the average recoreries（n =6） were 97.5% and 97.1% with RSD 2.8% and 1.7% respectively. Conclusion： The method is simple, rapid, accurate and suitable for the determination the contents of luteolin-7-o-glucoside and buddleoside in Chrysanthemum Indicum L.