凋亡抑制基因livin在食管癌中的表达

Expression of livin gene in esophageal cancer

目的:探讨凋亡抑制蛋白livin在食管癌组织中的表达及其与食管癌临床病理的相关性。方法:采用RT-PCR结合银染技术检测食管癌组织中livin-α mRNA和livin-β mRNA的表达。结果:36例食管癌组织中livin表达阳性率为50%,其表达与癌组织浸润深度和淋巴结转移有关(P<0.05),而与癌组织分化程度无关(P>0.05)。18例癌旁正常食管组织中livin低表达,阳性率为5.6%,与食管癌组织比较差异十分显著(P<0.01)。结论:livin在食管癌组织中异常表达,有望成为食管癌诊断和基因治疗的新靶点。

Objective：To study the expression of livin in esophageal cancer, and to explore its relationship with the clinical pathology of esophageal cancer. Methods：The expressions of livia-α and livin-β mRNA were detected by reverse transcription polymerase chain reaction combined with silver staining technique in 36 patients with esophageal cancer. Results：The positive expression rate of livin mRNA in the tumor tissues was 50.0%. The expression of livin was correlated with the depth of invasion and lymph node metastasis （P 〈 0.05 ） ,not the histological grade. The positive expression rate of livin in the para-cancerous tissues was 5.6%. Conclusion：Aberrant expression of livin may be a new target for the diagnosis and gene treatment of esophageal cancer.