蛋白激酶B对小鼠1-细胞期受精卵卵裂的影响

Effect of Protein Kinase B on Cleavage of Mouse Fertilized Eggs

目的:研究蛋白激酶B(PKB)的活性变化对小鼠受精卵早期发育的影响。方法:将野生型、持续激活型和激酶失活型PKB克隆至pBluescriptII/SK表达载体中,体外转录成mRNA,显微注射到1-细胞期受精卵中,观察受精卵分裂情况。结果:1-细胞期受精卵注射野生型或持续激活型PKB的mRNA后,卵裂率均增加,而持续激活型PKB的促进作用更为显著;相反注射激酶失活型PKB的mRNA后,卵裂率明显降低。结论:PKB能促进小鼠1-细胞期受精卵的发育,其活化对于小鼠受精卵的发育是必需的。

Objective： To investigate the effect of protein kinase B（PKB） on the regulation of mouse early embryo development. Methods： Wild type, constitutively active and kinase dead PKB（PKB-KD） were cloned and inserted into the vector pBluescript II/SK, transcribed into mRNA in vitro and then microinjected into mouse 1-cell stage fertilized eggs to observe the mitotic division of eggs. Results： Injection of mRNA coding for a constitutively active PKB（myr-PKB） into 1-cell stage fertilized eggs induced cell division more effectively than injection of wild type PKB（PKB-WT） mRNA, whereas microinjection ofmRNA of PKB-KD delayed the first mitotic division. Conclusion： PKB can promote the division of mouse 1-cell stage fertilized eggs. The activation of this kinase is necessary for the first round mitosis of mouse embryos.