沃氏甲烷球菌利用CO产CH_4代谢途径的研究

The research on metabolic rout of M.voltae converting CO to CH_4

对沃氏甲烷球菌(M.voltae)利用CO产CH4的代谢途径进行了研究.结果表明:该菌能在缺乏乙酸盐的培养基中合成生长所需的乙酸盐.并且在纯CO试验组中检测到有中间代谢产物H2和CO2的生成,而在H2/CO试验组中没有检测到中间代谢产物CO2.该菌在利用H2/CO和纯CO时都生成中间代谢产物甲酸盐.另外,还发现该菌利用CO产CH4的最适CO浓度为25%.在此CO浓度下,该菌利用H2/CO的平均产甲烷速率为1.98μmol.mg-1(细胞湿重).h-1;利用纯CO的平均产甲烷速率为0.42μmol.mg-1(细胞湿重).h-1.当CO浓度大于25%时,该菌利用H2/CO和纯CO产CH4都受到抑制,但H2/CO实验组受到抑制的程度明显小于纯CO实验组.

The experiment concerned the metabolic rout of M. voltae converting CO to methane. The result indicated that M. voltae was capable of synthesizing the acetate in the medium without acetate. CO2 and H2 were detected in medium with pure CO; however, in medium with H2/CO, no CO2 was detected during methane formation. More over, it resulted that M. voltae could utilize both H2/CO and pure CO to produce formate. Besides, the optimum CO concentration of M. voltae growth resulted to be 25 %. The average CH4 output of using H2/CO was 1.98μmol·mg^-1 （fresh cell weight）· h^-1, and the average CH4 output of using pure CO was 0.42μmol· mg^- 1 （fresh cell weight）· h^-1. When the concentration of CO was higher than 25 %, the methane production was inhibited when using H2/CO and pure CO, however, the inhibition of using H2/ CO is obviously lower than that of using pure CO.