摘要
目的研究胃窦肌间神经丛中单胺氧化酶mRNA表达,探讨5羟色胺(5-HT)在胃起搏机制中的作用。方法通过手术建立Wistar大鼠胃起搏模型(近远端胃各缝制一对电极),分为起搏组(n=10)和对照组(n=6)。采用逆转录聚合酶链反应(RT-PCR)检测起搏组和对照组胃窦肌间神经丛中MAO-A mRNA、MAO-B mRNA的表达量,以恒定表达的β-actin作为内参照。计算MAO-AmRNA、MAO-B mRNA与β-actin mRNA表达积分光密度值的比例(MA/B,MB/B),以反映组织中MAO-AmRNA、MAO-B mRNA的相对表达量。结果RT-PCR研究显示起搏组MAO-A mRNA表达显著弱于对照组,起搏组MA/B比值明显较对照组较少(0.37±0.11vs0.95±0.57,P<0.001);而起搏组MAO-B mRNA表达与对照组无差别,两组间MB/B比值比较也无显著性意义(0.97±0.24vs1.01±0.58,P>0.05)。结论胃起博后胃肌间神经丛内单胺氧化酶mRNA表达量明显减少,表明胃窦肌间神经丛内5-HT可能在胃起搏中发生了重要作用。
Objective To study the expression of MAO mRNA in the antral myenteric plexus and to evaluate the role of 5-HT in the mechanism of gastric pacing. Methods The Wistar rat models with two pairs of electrodes were established by operation and were divided into two groups of rats including the pacing group（ n = 10） and the control group（ n =6）. Using reverse transcriptase polymerase chain reaction（RT-PCR） ,the expression levels of MAO-A mRNA, MAO-B mRNA in antral myenteric plexus were quantified in the pacing group and the control group. β-actin, a steadily expressed gene was used as the inside reference. The ratio of MAO-A mRNA, MAO-B mRNA to β-actin mRNA （MA/B, MB/B） in the integrated optical density were calculated to represent the relative expression amount of MAO-A, mRNA, MAO-B mRNA in the tissues. Results RT-PCR study showed the levels of MAO-A mRNA expression in the pacing group were much lower than that of the control, MA/B in pacing group was significantly decreased than that of the control（0. 37 ＋0. 11,0. 95 ＋0. 57, P 〈0. 001 ）. But there was no difference between the levels of MAO-B mRNA expression in the pacing and control groups and neither was MB/B between the pacing and control group（0. 97 ＋0. 24 vs 1. 01＋ 0. 58, P 〉0. 05 ）. Conclusion After gastric pacing, MAO mRNA expression is much lower in antral myenteric plexus, suggesting 5-HT may play an important role in the mechanism of gastric pacing.
出处
《临床消化病杂志》
2007年第5期289-291,共3页
Chinese Journal of Clinical Gastroenterology
基金
国家自然科学基金资助项目(NO.30170350)
