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小鼠卵母细胞去核方法的初步研究 被引量：1

Preliminary Study on the Enucleation Method of Mouse Oocyte
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摘要 [目的]寻求既简单又有效的提高小鼠卵母细胞去核率的方法。[方法]分别采用3种不同的方法(盲吸法、点击法、负压-点压法),研究对MII期小鼠卵母细胞去核率的影响。[结果]结果表明,采用负压-点压法去核,其卵母细胞的去核率可达62.8%,与其他两种方法所获得的去核率差异显著(P<0.05)。[结论]负压-点压法是一种简单、有效的小鼠卵母细胞去核方法。 [Objective] This experiment is to explore simple and effective method to improve mouse oocyte enucleation rate. [Method] Mouse MII oocytes were enucleated by three methods： Blind-sucking, point-hitting and negative-pressing and point-pressing, [Result] The rate of enucleation was 62.8 % by negative-pressing and point-pressing, and the difference were significant as compared with other groups （P〈0.05）. [Conclusion] negative-pressing point-pressing method is a simple and effective method for mouse oocyte enucleation.
作者 淡新刚 史远刚
机构地区 宁夏大学农学院
出处 《安徽农业科学》 CAS 北大核心 2007年第32期10305-10306,共2页 Journal of Anhui Agricultural Sciences
关键词 卵母细胞 去核 负压-点压法 Oocytes Enucleation methods Negative-pressing point-pressing method
分类号 Q954.43 [生物学—动物学]
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参考文献6

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共引文献23

同被引文献16

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引证文献1

安徽农业科学
2007年 第32期

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