长寿花离体快繁研究

Study on in Vitro Rapid Propagation of Kalanchoe blossfeldiana

[目的]研究长寿花离体快繁的培养基配方和培养条件。[方法]以长寿花的幼嫩叶片作为外植体,以MS为基本培养基,诱导出不定芽进行快速繁殖,并比较添加4种浓度的BA和NAA对长寿花不定芽的诱导和生根效果的影响。[结果]长寿花幼嫩叶片的诱导和生长与生长素、细胞分裂素2种激素有关系,两者的配比直接影响了叶片的再生频率。长寿花叶片诱导和不定芽增殖的最适培养基为MS+6-BA1.0 mg/L+NAA0.5 mg/L,最适生根培养基为1/2 MS+NAA0.5 mg/L,不定芽在此培养基上的生根率最高,移栽成活率为95%。通过直接诱导可获得较高的不定芽诱导率,同时增殖培养的增殖系数达到10,大大缩短了常规育苗时间。[结论]该研究建立了长寿花组织培养的再生体系,为其快速繁殖及大规模的工厂化育苗提供科学依据。

[Objective] The aim of the research was to study the medium formula and culture conditions for in vitro rapid propagation of Kalanchoe blossfeldiana. [Method] With tender leaves of K. blossfeldiana as explant and MS as basic medium, adventitious buds were induced for rapid propagation. And the effects of adding 4 concentrations of BA and NAA on the induction of adventitious buds and rooting effect of K. blossfeldiana were compared. [Result] The induction and growth of tender leaves in K. blossfeldiana had relations with 2 kinds of hormone including auxin and cytokinin, and the matching of auxin and cytokinin had a direct effect on the regeneration frequency of leaves. The optimum medium for the induction of leaves and the proliferation of adventitious buds was MS＋6-BA1.0 mg/L ＋NAA 0.5 mg/L and the optimum rooting medium was 1/2 MS＋NAA 0.5 mg/L on which the adventitious buds obtained highest root rate with their transplanting surviving rate of 95 %. By direct induction, higher inducement rate of adventitious buds could be obtained and propagation coefficient of propagation culture reached 10, which shortened the time of conventional seedling. [Conclusion] The research established the regeneration system of tissue culture in K. blossfeldiana and provided scientific basis for its rapid propagation and factory-cultivated seedling in large scale.