蒲包花种子组织培养的研究

Study on the Tissue Culture of Calceolaria crenatiflora Seeds

[目的]摸索蒲包花种子组织培养技术,解决蒲包花种子播种繁殖率低的问题。[方法]以蒲包花种子为外植体,采用不同的消毒方式、培养基和基质,研究了蒲包花的组织培养。[结果]经浓度70%酒精消毒30 s,蒲包花种子污染率较低,为18.7%,萌发率高,达62.5%。蒲包花不定芽的最佳培养基为MS+BA1.0 ml/L+IAA0.1 ml/L,增殖系数达5.0,丛生芽多,且生长健壮;蛭石+珍珠岩基质对植株的扦插最好,成活率达到63.0%;不经生根培养的试管苗的扦插成活率较高,可达60.0%,经生根培养的试管苗的扦插成活率为48.0%。[结论]IAA对蒲包花不定芽增殖的作用很明显,蒲包花试管苗可不经生根培养直接扦插。

[Objective] The study aimed to grope the tissue culture techniques of Calceolaria crenatiflora seeds so as to solve the problem of low sowing propagation rate of C. crenatiflora. [Method] With C. crenatiflora seeds as explants, the tissue culture of C. crenatiflora was studied with different sterilization methods, media and stroma. [Result] Through sterilizing with 70% alcohol for 30 s, the contamination rate was lower, being 18.7 % and the germination rate was higher, being 62.5 %. The optimum medium for C. crenatiflora adventitious buds was MS ＋ BA 1,0 ml/L ＋ IAA 0.1 ml/L. The multiplication coefficient was up to 5.0. The plantlets grew vigorously with abundant cluster buds. The stroma of vermiculite ＋ perlite was best for the cutting of plantlet, with the survival rate up to 63.0 %. The tube plantlets through no rooting culture had higher cutting survival rate, reaching 60.0 %, and that through rooting culture had cutting survival rate of 48.0 %.[Conclusion] IAA had very obvious effect on the multiplication of C. crenatiflora adventitious buds. The tube plantlets of C. crenatiflora could be made for direct cutting through no rooting culture.