工频磁场对人绒毛滋养细胞凋亡相关基因表达的影响

Apoptosis-related gene expression of human villous trophoblasts exposed to 50 Hz magnetic field

目的研究50 Hz工频磁场对人早孕绒毛滋养细胞凋亡相关基因表达的影响,探索工频磁场对人生殖健康影响的可能机制。方法体外分离培养人早孕绒毛滋养细胞,以50 Hz、0.4 mT强度的正弦磁场分别辐照处理6、48、72 h,同时设立相应的平行对照组,用实时荧光定量逆转录—聚合酶链反应(RT-PCR)方法检测工频磁场辐照对bcl-2、bax、caspase-3、p53、fas基因表达的影响。结果辐照6、48、72 h后bcl-2、bax、caspase-3、p53、fas基因表达变化倍数平均值均接近1,暴露组与对照组的差异均没有统计学意义(P<0.05)。结论72 h内,0.4 mT工频磁场体外辐照不会影响人早孕绒毛滋养细胞bcl-2、bax、caspase-3、p53、fas等凋亡相关基因的表达水平。

Objective To study apoptosis-related gene expression of human villous trophoblasts exposed to 50 Hz magnetic field and to investigate the possible mechanism of human reproductive health effects caused by 50 Hz magnetic field. Methods Cultured human villous trophoblasts were exposed to 50 Hz magnetic field at 0.4 mT for 6, 48, 72 hours. Gene expressions of Bcl-2, Bax, Caspase-3, p53 and Fas were analyzed using real-time reverse transcription polymerase chain reaction （RT-PCR） assay. Results Within 72 hours, the average fold change for each gene was near 1.00, and there was no significant difference on expression pattern in each gene between exposure and control groups （P〉0.05）. Conclusion 0.4 mT 50 Hz magnetic field does not affect the apoptosis-related gene expression of human villous trophoblasts in vitro.