高效液相色谱法同时测定食品中安赛蜜、苯甲酸、山梨酸、糖精钠以及脱氢乙酸

Simultaneous determination of acesulfame,benzoic acid,saccharin acid and dehydroacetic acid in foods by high-performance liquid chromatography

建立食品中安赛蜜、苯甲酸、山梨酸、糖精钠、脱氢乙酸等甜味剂和防腐剂的高效液相色谱测定法。方法采用C18(5um,4.6mm×150mmi.d)反相色谱柱,流动相:v(甲醇)∶v(0.02moL/L乙酸铵溶液)=(5∶95),流速1.0mL/min,检测波长230nm,柱温30℃,进样量5μL。样品的检出限1.5～4.4mg/kg,线性范围1～10mg/L,加标回收率95.5%～103.6%,相对标准偏差为0.95%～2.81%(n=6)。该法具有样品预处理简单,灵敏度高,分析时间短等优点,可用于食品样品的直接测定。

HPLC is applied to the separation and determination of acesulfame, benzoic acid, saccharin, saccharin and dehydroacetic acid in foods. ODS C18 is used as the analysis column, and a solution, containing methanol and 0.02mol/L acetic ammonium solutiong mixed in the ratio of 5 to 95, is used as the mobile phase. The flow is 1.0mL/min, the detect wavelength is 230nm, the column temperature is 30℃, and the injection volume is 5μL. The limit of detection is between 1.5mg/kg and 4.4mg/kg, the linear plots were obtained between 1 and 50mg/ L. The fortification recoveries are between 95.5% and 103.6%, and RSD （n=6）value are between 0.95% and 2.81%. This method with high sensitivity, simple and rapid in pretreatment, can be used in foods samples directly.