摘要
For studying on pathogenicity mechanism of Fusarium moniliforme,REMI was used to transform protoplasts of FT1 strain with the vector pUCATPH,which contained hygromycin B-resistant gene.More than 300 transformants had been obtained,most of them were quite stable after five rounds of successive culture.25 mutants of morphology and 2 weak pathogenicity mutants were gained by REMI.PCR amplification showed that the hygromycin B-resistant gene had integrated into genomes of the two pathogenicity mutants.The optimum conditions of preparing protoplasts were: the mycelia growing in PDB medium for 14 h,lywallzyme was used to digest the mycelia at 100 r/min,30℃ for 4 h,and 0.7 mol/L NaCl was used as the osmotic stabilizer.
For studying on pathogenicity mechanism of Fusarium moniliforme, REMI was used to transform protoplasts of ET1 strain with the vector pUCATPH, which contained hygromycin B-resistant gene. More than 300 transformants had been obtained, most of them were quite stable after five rounds of successive culture. 25 mutants of morphology and 2 weak pathogenicity mutants were gained by REMI. PCR amplification showed that the hygromycin B-resistant gene had integrated into genomes of the two pathogenicity mutants. The optimum conditions of preparing protoplasts were : the mycelia growing in PDB medium for 14 h, lywallzyme was used to digest the mycelia at 100 r/min, 30℃ for 4 h, and 0.7 mol/L NaCl was used as the osmotic stabilizer.
出处
《植物病理学报》
CAS
CSCD
北大核心
2007年第5期545-548,共4页
Acta Phytopathologica Sinica
基金
国家自然科学基金资助项目(30571246)