蒿甲醚对不同病期小鼠结直肠癌抑瘤效果的实验研究

Experiment of inhibitive effect of artemether in different stages on colorectal cancer growth in BALB/c mice

目的:探讨蒿甲醚(Artemether)对不同病期BALB/c小鼠CT-26结直肠癌的抑瘤作用。方法:BALB/c小鼠皮下接种CT-26结直肠癌细胞(4×105个)。96只小鼠,雌、雄各半,随机分为两个大组,分为中期组(接种5天后)和晚期组(接种10天后);每个大组再随机分为6小组,每组8只,分别为低剂量(33.3mg/kg)、中剂量(50mg/kg)、高剂量(66.6mg/kg)和中剂量(50mg/kg)加铁剂(1.5mg/kg)组、阳性对照组(顺铂5mg/kg)、空白对照组(等体积生理盐水)。结果:中期组口服蒿甲醚高、中、低剂量和中剂量加铁剂对BALB/c小鼠CT-26结直肠癌均有明显的抑制作用,抑瘤率分别为52.0%、51.4%、42.3%和53.5%;晚期组口服蒿甲醚高、中、低剂量和中剂量加铁剂对BALB/c小鼠CT-26结直肠癌亦有明显的抑制作用,抑瘤率分别为35.8%、33.5%、38.2%和44.7%,中剂量单药抑瘤率最低;中、晚期各对应组之间比较有显著差异(P<0.05)。结论:在一定剂量范围内,口服蒿甲醚对小鼠不同病期的CT-26结直肠癌均有明显的抑制作用,中期组强于晚期;蒿甲醚与顺铂相比,具有较小的毒副作用;与铁剂合用具有一定的协同作用,使抑瘤率增加。

Objective：To explore the inhibitive effect of artemether （Art） in different stages on colorectal cancer growth in tumor bearing BALB/c mice. Methods：Ninty-six BALB/c mice were divided two groups that one group was middle clinic stage and another group was advanced stage, each group was 48 BALB/c mice which were subcutaneous planted with CT-26 colorectal cell （4×10^5） were divided resourcefully into 6 groups, each group was 8 mice. Group 1, Art 33.3mg/kg （low dose） was oral; group 2 Art 50mg/kg （ middle dose） oral; group 3 Art 66.6mg/kg（ high dose） oral; group 4 ferralia 1.5mg/kg and Art 50mg/kg （ middle dose） oral; group5 DDP 5mg/kg oral as positive control and 7 normal saline oral as blank control. Results：In the middle stage, inhibitive rates of Art by oral at dosages of low, middle and high were 42. 3%, 51.4% and 52. 0% respectively, and had significant difference of inhibitive effects on colorectal growth （ P = 0. 007, P = 0. 006, P = 0.024, respectively）. In the advanced stage, inhibitive rates of Art by oral at dosages of low, middle and high were 35.8%, 33.5% and 38. 2% respectively, the dosage of low and high had significant difference of inhibitive effects on colorectal growth （ P 〈 0. 05）, dosage of middle had no significant difference of inhibitive effects on colorectal growth （P = 0. 07）. Combination ferralia and Art synergenic the inhibitive effect on tumor bearing mice. Conclusion： Artemether has inhibitive effect to colorectal cancer growth in mice at the range of dosage and combination ferralia and artemether synergenic has the inhibition effect; it has a significant and different therapy efficacy in different stages of the disease.