100例骨髓转移癌骨髓组织病理学的观察

Observation on histopathological changes in 100 cases of bone marrow metastatic cancer

目的:观察100例骨髓转移癌患者骨髓组织病理学的改变,并与骨髓涂片结果进行比较。方法:100例骨髓转移癌患者骨髓检查,行一步法骨髓抽吸取材,石蜡或塑料包埋骨髓组织,切片2μm或3μm厚作HGF或MGG染色,切片5μm厚作Gomori染色,必要时作Masson三色染色,按三种方法各染色1张切片。骨髓涂片行瑞特染色。结果:活检切片的诊断阳性率及判断骨髓增生度等较涂片明显为优。胶原纤维Masson三色染色呈阴性,提示骨髓转移癌并发骨髓纤维化,绝大多数为网硬蛋白型。结论:骨髓活检可作为诊断骨髓转移癌的常规检测手段,能够提高诊断率及评估疗效和预后。

Objective：We observed the histopatholgical changes of biopsy sections of bone marrow in 100 cases of bone marrow metastatic cancer and compared them with that of bone marrow smears. Methods：The paitents＇ bone marrow with bone marrow metastatic cancer were aspirated in one step. Paraffin or plastics pack cover up marrow organization, HGF or MGG, Gomori and Masson dye a slice; Wright dye morrow slice. Results：The results showed the bone marrow biopsy was obviously more superior in increasing the correct rate of diagnosis and in iudgement of the degree of myeloidosis than smear examination, In addition ,the accumulated marks of reticulin increased markedly than that of normal group （ P 〈 0. 01 ）, but it was negative with Masson stain,showing that most of bone marrow metastatic cancer with myeloidosis was reticulin type. Conclusion：Bone marrow biopsy was used to diagnosis bone marrow metastatic cancer. It can improve the rate of diagnosis and assess the medical treatment and the prediction.