谷胱甘肽S转移酶基因多态性对1-溴丙烷接触者神经传导速度的影响

The Association between GSTT1 and GSTM1 Gene Polymorphism and Nerve Conduction Velocity in Workers Exposed to 1-Bromopropane

[目的]探讨谷胱甘肽S转移酶基因多态性对1-溴丙烷(1-BP)接触者神经传导速度的影响。[方法]选择116名1-BP接触工人为接触组,133名无1-BP接触史的工人为对照组,用神经电生理检测技术测定工人的运动神经传导速度(MCV)、感觉神经传导速度(SCV)和末端潜伏期(DL),用等位基因特异性扩增(ASA)方法检测两组GSTT1和GSTM1基因型的分布。[结果]GSTT1基因多态性和1-BP接触对工人的SCV和DL的影响有交互作用,SNK两两比较结果显示,与对照组GSTT1基因携带者相比,接触组中GSTT1基因缺失型工人的SCV明显减慢[分别为(45.3±5.0)m/s和(40.1±5.3)m/s]、DL明显延长[分别为(6.3±1.3)ms和(8.3±2.0)ms],差别有统计学意义(P<0.05);去除交互项后的两因素方差分析显示,GSTT1基因多态性对工人的MCV有独立的影响(P<0.05)。未发现GSTM1基因多态性对接触者外周神经传导速度有影响。[结论]GSTT1基因多态性与接触者对1-BP外周神经毒性的易感性有关。

[ Objective ] To explore the association between GSTT1 and GSTM1 gene polymorphism and nerve conduction velocity in workers exposed to 1-bromopropane（ 1-BP ）. [ Method ] Neuroelectrophysiological studies were carried out on 116 1-BP exposed and 133 nonexposed workers. The polymorphism of GSTT1 and GSTM1 were analyzed by allele specific amplification（ ASA ） method. [ Result ] GSTT] gene polymorphism and exposure had an interact effect on sensory nerve conduction velocity（ SCV ） and distal latency（ DL ）. Workers with GSTT1 gene null type in exposed group had a slower SCV[ （ 40.1 ±5.3 ）m/s ]and longer DL [ （ 8.3 ±2.0 ）ms ]compared with those with GSTT1 gene normal type in nonexposed group[ SCV（ 45.3 ± 5.0 ）m/s, DL（ 6.3 ±1.3 ） ms ], P 〈 0.05. GSTT1 gene polymorphism also had an effect on motor nerve conduction velocity（ MCV ）. There is no evidence that GSTM1 gene polymorphism had effect on nerve conduction velocity of these workers. [ Conclusion ] It suggested that GSTM1 gene polymorphism might be associated with the susceptibility to neurotoxicity of 1-BP.