血管抑制基因治疗对增生性瘢痕的病理学影响

The pathological influence of antiangiogenesis gene therapy on hypertrophic scar

目的:研究血管抑制基因治疗对兔耳增生性瘢痕的病理学影响。方法:复制兔耳增生性瘢痕,待创面上皮化后10天瘢痕组织局部多点注射基因重组血管抑制剂Ad-METH1(血管生成抑制因子-1,extracellular protein with metalloprotease and thrombospondin1 domains),30天后观察兔耳瘢痕组织形态、超微结构的变化。结果:Ad-METH1注射后30天,兔耳瘢痕组织学染色显示微血管分布明显减少,超微结构显示内皮细胞肿胀、变性,部分血管丧失功能,成纤维细胞增殖及分泌活性明显降低。结论:Ad-METH1对增生性瘢痕的形成有明确的抑制作用,血管抑制基因治疗有望为增生性瘢痕的防治提供新的方法。

Objective To investigate the pathological influence of antiangiogenesis gene therapy on hypertrophic scar of rabbits＇ ears.Methods The hypertrophic scar of rabbits＇ ears was reproduced.At the 10th day after epithelization on the surface of wound,Ad-METH1 was injected into parts of scar tissue on different points.30 days later,changes of structure and ultrastructure of scar tissue of rabbits＇ ears were observed.Results At the 30th days after injection,H＆E deying of the hypertrophic scar of rabbits＇ ears showed that the distribution of micrangium declined distinctly.Ultrastructure revealed that endotheliocytes were tumefied and destructured,some blood vessels lost functions and proliferation and secretion of fibroblast reduced markedly.Conclusion Ad-METH1 has marked inhibitory function on the form of hypertrophic scar.Antiangiogenesis gene therapy could provide a new method to prevent and cure hypertrophic scar.