钙离子在P物质促胃癌细胞增殖过程中的作用

Role of Ca^(2+) in the proliferation of gastric cancer cells by substance P

目的:研究P物质(SP)对体外培养的人低分化胃癌细胞系MKN45增殖的调节作用,探讨钙信号在其中的作用.方法:以第4代人胃癌细胞系MKN45为研究对象,用MTT方法观察了不同浓度的SP,ASN-1377642(NK-1受体拮抗剂)、新霉素(PIP2拮抗剂)、尼卡地平(钙通道阻断剂)对MKN45增殖的影响.结果:对于体外培养的人低分化胃癌细胞系,不同浓度的SP(10,50,100nmol/L)可以显著的刺激MKN45的增殖,其增殖率分别为57.23%,63.75%,52.95%(P<0.05).不同浓度的ASN-1377642、尼卡地平和新霉素与MKN45预先孵育后SP促MKN45的增殖率显著降低(P<0.05),但是增加上述药物剂量其抑制效应并无显著增加(P>0.05).结论:SP可通过与NK-1结合有效促进胃癌细胞增殖.ASN-1377642,尼卡地平和新霉素可以显著的抑制SP的促增殖效应,Ca2+可能在该过程中起重要作用.

AIM： To investigate the proliferation effect of sub- stance P （SP） on gastric cancer cells, and the role of calcium signal during this process. METHODS： Fourth-generation MKN45 cell line was used in the study. MTT colorimetry was used to determine the impact of SP, ASN-1377642 （a specific antagonist of NK-1 receptor）, neomycin （PIP2 antagonist） and nicardipine （calcium channel blocker） on the proliferation of gas- tric cancer cell line MKN45. RESULTS： In vitro, different con- centrations of SP （ 10, 50, 100 nmol/L） significantly promoted the proliferation of MKN45 cell line （proliferation rate： 57.23%, 63.75% , 52.95% , P 〈 0.05）. Different concentrations of ASN- 1377642, neomycin and Nicardipine significantly inhibited the SP enhanced proliferation of MKN45 cells （P 〈 0.05 ）, but the inhi- bition effects of ASN-1377642, neomycin and nicardipine were not enhanced when their concentrations were increased （P 〉 0.05 ）. CONCLUSION ： SP can induce the proliferation of gastric cancer cell line MKN45 through binding with its receptor NK- 1. ASN-1377642, neomycin and nicardipine can inhibit this effect. Calcium may play an important role during this process.