TNF-α基因多态性与分泌性中耳炎的关联

The association of gene polymorphism of tumor necrosis factor-alpha with secretory otitis media

目的探讨TNF-α基因启动子-238G/A多态性与分泌性中耳炎(SOM)的相关关系。方法应用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测125例SOM患者和130例健康对照者TNF-α基因启动子- 238G/A多态性。结果TNF-α-238基因型GG、GA频率在对照组和SOM组分别为93.85%,6.15%和85.60%,14.40%;等位基因G、A频率在对照组和SOM组分别为96.92%,3.08%和92.80%,7.20%。两组差异均有显著性(P<0.05),携带GA基因型个体患SOM的风险约是GG基因型的2.565倍(OR=2.565,95%CI=1.072～6.138)。结论TNF-α-238基因多态性与SOM的发病有相关性,A等位基因可能是SOM的遗传易感基因。

Objective To examine the association between polymorphism of tumor necrosis factor- alpha （TNF-alpha） -238 G/A promoter with secretory otitis media （SOM）. Methods The polymorphlsm of TNF- alpha was detected by polymerase chain reaction - restriction fragment length polymorphism （PCR- RFLP） methods in 125 patients with SOM and 130 healthy controls. Results TNF- alpha genotype frequencies of GG, GA were 93.85 %, 6.15 % and 85.60 %, 14.40 % in SOM group and control group, respectively. Allele frequencies of G, A were 96.92 %, 3.08 % and 92.80 %, 7.20 % in SOM group and control group, respectively. There was statistically significant difference in frequencies Of allele and genotype in TNF-alpha polymorphism between SOM group and control group （ P 〈0.05）. The relative risk suffered from SOM in patients with GA genotype was as 2. 565 times as those with GG genotype （ OR = 2. 565, 95% CI = 1.072-6. 138）. Conclusion TNF - alpha- 238 polyrnor-phism is positively associated with the genesis of SOM, and A allele maybe a risk factor for SOM.