双黄败毒颗粒中黄连的薄层荧光扫描分析

Analysis on Rhizoma Coptidis in Shuanghuangbaidu Granules by TLC Fluorescence Scanning

应用薄层色谱荧光扫描法测定双黄败毒颗粒中小檗碱的含量并鉴别黄连药材。样品经提取、点样、薄层展开后，采用荧光扫描进行含量测定；展开剂为苯：乙酸乙酯：甲醇：异丙醇：浓氨试液=6：3：1．5：1．5：0．5（V／V）的混合液；激发波长λ=366nm。实验结果显示盐酸小檗碱的线性范围为0．041～0．369μg（R^2=0．9995），平均回收率为100．7％（RSD=2．56％），表明该法简便、快速、经济，可作为本制剂主药黄连的定性鉴别及小檗碱定量分析的有效方法。

TLC fluorescence scanning method was established for the determination of berberine and identifying of Rhizoma Coptidis in Shuanghuangbaidu granules. The sample was extracted and developed, the berberine hydrochloride was determinated by TLC fluorescence scanning with benzene： ethylacelate： methanol： isopropabol： ammonia solution =6： 3： 1.5.1.5： 0. 5（V/V） as a developing solvent system and 366 nm as scanning wavelength. A good linearity was shown in the range of 0. 041 ～ 0. 369 p.g （R^2 = 0. 999 5 ）, the average recovery was 100.7% （RSD = 2.56% ）. This method is simple, rapid and economical for the quality control of Shuanghuangbaidu granules.