鸡传染性法氏囊疫苗株B87的最佳收获时间和接种浓度

Detection of Optimal Ingathering Time and Inoculability Concentration of Infectious Bursal Disease Vaccine Strain B87

将鸡传染性法氏囊病中等毒力株B87株进行1：10、1：60、1：100及1：1000稀释，分别接种于鸡胚的尿囊腔中，分别于60～72、78～86、96和120h收获死胚，测定收获病毒液的效价。对比试验结果表明，1：60稀释、78～86h收获的死胚，IBDVB87株病毒含量最高。

The infectious bursal disease intermediate virus strain B87 was inocubated to the allantoic sack of chick embryo, the dead embryo were collected at 60 -72h,78 -86h,96h and 120h, the titer of B87 was determined. The result of contrast experiment showed the titer of dead B87 embryo collected at 78 - 86h from the B87 diluted to 1：60 was the highest.