摘要
目的 建立同时测定复方麝香草酚滴耳液中麝香草酚和水杨酸含量的方法。方法 采用高效液相色谱法。色谱柱为Shim-pack VP-ODS（4．6×150mm，5μm），流动相为甲醇-水-磷酸（60；40：0．3），检测波长220nm，柱温30℃。结果 水杨酸和麝香草酚分别在0．12～0．28mg·mL^-1和0．06～0．14mg·mL^-1范围内线性关系良好，平均回收率分别为101．2％（RSD=0．57％）和100．1％（RSD=0．64％）。结论 该方法可用于复方麝香草酚滴耳液的质量控制。
OBJECTIVE To establish a method for the determination of thymol and salicylic acid in complex thymol ear drops. METHODS The column was shim-pack VP-ODS（4.6× 150mm,5μm） ,the mobile phase consisted of methanol-water-phosphoric acid（60 ： 40 ： 0.3）, the detection wavelength was 220nm, the column temperature was 30℃. RESULTS There was a good linear relationship when the content of salicylic acid and thymol was in the range of 0. 12-0.28mg . mL^-1 , and 0.06 -0. 14 mg . mL^ -1 respectively. The average recoveries of salicylic acid and thymol were 101.2 %（ RSD = 0.57% ） and 100. 1% （ RSD =0.64%） ,respectively. CONCLUSION The method Can be applied to the quality control of complex thymol ear drops.
出处
《齐鲁药事》
2007年第10期599-601,共3页
qilu pharmaceutical affairs
