新疆和田地区维吾尔族JC病毒基因型的地理分布

Geographical distribution of JC virus genotypes of the Uygur nationality in Hetian region,Xinjiang

对新疆和田地区7个县的主体民族维吾尔族的JC病毒IG区域基因型的地理分布状况进行了对比研究。从178个40岁以上的男性尿样提供者中分离出了JC病毒,并且对它们进行了DNA提取,对JC病毒IG区域进行了PCR扩增和DNA序列测定,得到了117人的JC病毒DNA IG区域的碱基序列。多态分析研究结果表明,117人中的基因型分别由B1-b,B1-c和EU组成,各基因型所占比率为:88.9%,3.4%和7.7%。研究发现,在各基因型中B1-b所占的比率高,其次是B1-c和EU基因型。通过NJ法进行了聚类分析法构建分子系统树,分析发现该人群JC病毒基因型中广泛分布于欧亚大陆中心的B1-b型占优势。这个结果与维吾尔族的祖先是公元前3世纪在中国北方蒙古高原生居过的丁零民族的后裔这个历史事实是一致的。

The geographical distribution of genotype in IG region of JC virus of Uygur nationality was studied comparatively in seven Counties of Hetian region, Xinjiang. JC virus was separated from 178 samples of male Uygur donors who are over 40 years old, and DNA was extracted from these samples, after enlarging PCR and measuring DNA sequence of JC virus in IG region, and the base sequence of JC virus in IG region of 117 people was obtained finally. Polymorphic analysis results show that the base sequence of 117 people consists of B1 - b, B1 - c and EU 3 genotypes and the percentages are 88.9 percent, 3.4 percent, and 7.7 percent respectively. Through construction of molecule system tree, Genotype B1 -b was found in most of the sampled areas and was dominant in Hetian region. The base sequence analysis represents there is comparability between Genotype B1 - b in Hetian and that of Mongolian Highland, which linked the Uygur people to the Mongolian populations even as history shows the ancestors of Uygur nationality had been the descendants of certain minority inhabited in the Mongolian Highland about the third century BC. The difference between the Genotype B1 - c and EU in Hetian and those of Europe and West Asia proves Uygur nationality are remote descendants of European race populations. The Uygur people with Genotype B1 -c and EU in Hetian maybe immigrate from Europe and West Asia.