噻酮对离体乳猪皮肤光透明效果影响的实验研究

Experimental Study on influence of Thiazone on Optical Clearing of Piglet Skin in Vitro

研究一种新型促渗剂——噻酮对皮肤组织光透明效果的影响。分别用单纯聚乙二醇400(PEG400)溶液以及PEG400与噻酮的混合溶液作用于含角质层及去角质层的离体乳猪皮肤,利用积分球系统动态检测样品对632．8 nm光的透射率变化,并对样品拍照获得直观图。结果表明:混合溶液作用于两种皮肤模型后,其光透明性均有不同程度的增加,即衰减系数降低;比较去角质层皮肤与含角质层皮肤的衰减系数的变化,前者比后者大19倍;单纯的PEG400仅对去角质皮肤有一定的光透明效果,并且所导致的皮肤衰减系数的降低仅为混合液作用后的2/3。因此,噻酮作为良好的促渗剂能与PEG400产生协同效应,有效提高皮肤光透明效果。

Thiazone, a kind of new enhancer, is introduced to investigate the effect of optical clearing on skin tissue. The samples including skin with or without stratum corneum （SC）, are treated with pure polyethyleneglycol 400 and mixed solution （thiazone in combination with polyethyleneglycol 400） respectively. The transmittance of light with wavelength 632.8 nm is measured dynamically by using an integrating sphere system, and then photos of the samples are taken by CCD. Experimental results show that mixed solutions have optical clearing effect on both kinds of skin, The change in attenuation coefficient of skins without SC is 19 times of that with SC. Single PEG400 can only do some effect on the sample without SC, and the change in attenuation coefficient of samples is just 2/3 of that treated by mixed solution. It is illustrated that thiazone can increase the effect of optical clearing on skin, because it is an efficient enhancer and has synergistic effects with PEG400.