用HPLC法测定红酵母中β-胡萝卜素的含量

Studies of the Expression of Progesterone Receptor Gene in the Rat Uterus during Early Pregnancy by RT PCR

对从红酵母中提取β－胡萝卜素的方法进行了初步探讨，并以高压液相色谱（ＨＰＬＣ）方法测定了红酵母中β－胡萝卜素的含量，采用ＯＤＳ为固定相，以甲醇∶氯仿（７０∶３０）为流动相，检测波长为４５０ｎｍ，该方法准确、快速、可靠．平均回收率为９２．０％．

Progesterone plays an important role in the establishment and maintenance of pregnancy. However, there have been no systematic reports on the expression of the rat uterine progesterone receptor (PR) gene during this process. In the present studies PR mRNA levels in rat uterus were analyzed during the estrous cycle and early pregnancy (d1￣d10), using reverse transcription polymerase chain reaction (RT PCR). PR mRNA level was the highest at estrus and the lowest at metestrus, with the former value twice the latter. Post coitum PR transcription increased rapidly, reached a peak of two fold of the d1 level on d3￣d4 (before implantation), but drastically dropped to 50% of the d1 level on d6 (during implantation) and remained unchanged until d10. It is of interest to discover that the uterine PR mRNA level at the implantational sites was much higher than that at the non implantational sites on d7￣d10. Preliminary discussion was made on the physiological significance of these changes.