应用分子生物学技术检测布鲁氏菌抗原的研究——Ⅱ PCR技术在动物布鲁氏菌病早期诊断中的应用

DETECTION OF BRUCELLA USING MOLECULAR TECHNIQUES : II DIAGNOSIS OF ANIMAL BRUCELLOSIS BY PCR

用PCR技术诊断动物布病,还未见报道。作者首次用牛种布氏菌104M株100万1只的菌量感染普通小鼠,分别在感染第5d、10d 采血,同时做血清学反应和PCR检测为阳性,同时,还检查了感染15d的豚鼠,其SAT和PCR结果均为阳性,PCR和SAT在感染的后期有很高的符合率。

Mice were infected with B. abortus 104M 106/animal,then sera tests and PCR were performed after 5 and 10 days, the results showed that, there was no specific antibody within 10 days,while PCR tests were positive. At the same time,we tested 3 guinea pig which infected B. abortus 104M for 15 days,SAT and PCR results were also positive, PCR with SAT had high correspondence in the period of late infection.