动脉粥样硬化病变中p53基因的突变与脂质关系的研究

The relations between mutations of p53 anti oncogene atherosclerosis and levels of lipids in serum.

分析中国成人动脉粥样硬化（ＡＳ）病变中ｐ５３抑癌基因结构的突变与血清脂质水平及动脉管壁粥样硬化组织内的脂质含量之间的关系。方法应用α－３２Ｐ－ｄＣＴＰ底物标记的ＤＮＡ多聚酶链反应－单链构像多态性分析技术检测ｐ５３基因结构的突变；同时测定了ｐ５３基因突变组与无突变组的血清总胆固醇（ＴＣ）、甘油三酯（ＴＧ）、胆固醇酯（ＣＥ）及动脉管壁粥样硬化组织内的ＴＣ、ＴＧ、ＣＥ含量。结果ｐ５３基因突变组的动脉管壁硬化组织内的ＴＣ、ＴＧ、ＣＥ含量显著高于无突变组（Ｐ≤０．０５～０．０１）。两组间血清ＴＣ分别为５．９６±１．１４ｍｍｏｌ／Ｌ、５．４７±１．０９ｍｍｏｌ／Ｌ（Ｐ＞０．２５）ＴＧ分别为１．３０±０．２７ｍｍｏｌ／Ｌ、１．４２±０．２９ｍｍｏｌ／Ｌ（Ｐ＞０．０５）；结论ｐ５３抑癌基因结构的突变可能是动脉粥样硬化形成过程中的另一重要因素。

Objective To further understanding the pathogenesis of huamn atherosclerosis, we studied the mutations of p53 anti oncogene in Chinese atherosclerosis lesions and the relations between p53 gene mutations with the levels of serum lipoprotein and the contents of lipids in AS tissues. Methods p53 gene mutations were detected by a 32 P dCTP labelled radiative PCR SSCP and the biochemical analyses of lipids in serum and AS tissues. Results In 89 cases studied, 9 were found the p53 gene multi hotsports exons mutations. The TC, TG annd CE contents in vascular AS tissues in p53 gene mutation group were higher than those in non mutations group (ANOV: F=4.56～9.97, P <0.05～0.01, no differences in PL contents). But there were no statistic differences in serum levels of TC, TG, CE and apoAI between the two groups ( P >0.05～0.25). All the levels of the serum lipoproteins studied were almost in normal ranges. Conclusion The DNA structural mutations of p53 anti oncogene in Chinese adults′ AS tissues have remarkable relations with the severity of AS lesions. The mutations of p53 gene may play some important roles in the formation of human atherosclerosis. In addition to serum lipid disorders, this may be a new mechanism in the pathogenesis of human atherosclerosis.