P^53基因突变与乳腺癌关系的初步研究

MUTATION OF P53 GENE IN BREAST CANCER

应用聚合酶链式反应－单链构象多态性分析（ＰＣＫ－ＳＳＣＰ）法检测３０例乳腺癌组织中Ｐ基因的５、６、７３个外显子。结果显示，１５例有异常电泳带，表明这些病例相应的ＤＮＡ片段发生突变。结合临床分析发现：在（１）Ⅱ期７／１４例、Ⅲ期６／１１例、Ⅳ期２／２例，（２）浸润性导管癌１３／２６例、乳头状腺癌１／１例、导管内癌１／１例，（３）腋窝淋巴结阳性１１／１８例、腋窝淋巴结阴性４／１２例中发现Ｐ基因突变。提示Ｐ基因突变与临床分期及病理改变有关，有Ｐ５３基因突变的原发病灶具有较强的侵袭能力，易复发和转移。

The exon 5 , 6 , 7 of p53 gene in frozen breast cancer specimen was detected. Using PCR-SSCP method , the abnormal shifting of the single stranded DNA ( ssDNA ) was identified in 15 out of 30 cases. The distribution of mutation among these cases were 1. according to stage of diseaae , stage Ⅱ 7 / 14 , stage Ⅲ 6 / 11 , stage Ⅳ 2 / 2; 2 . according to pathological type , infiltrative ductal carcinoma 13/26 , papillary adeno carcinoma 1 / 1 , intraductal carcinoma 1 / 1 ; 3. According to axillary nodal status , positive axillary lympho node 11/18,megative axillary lympho mode 4/12.The result indicates that the mutation of p53 gene is intimatly related to clinical stage and pathological type . The carcinoma with positive p53 gene mutation in more invassive , recurred and metastaticl.