P物质及组胺受体拮抗剂对分泌性中耳炎中耳积液的影响

The effect of substance P upon middle ear effusion of secretory otitis media and the mode of action

采用免疫组化ABC－GDN及图象分析技术研究分泌性中耳炎（SOM）中耳粘膜P物质（SP）含量的变化与中耳积液（MEE）的关系，并观察SP受体措抗剂spantide及组胶H2受体阻滞剂cime－tidin对MEE的影响，探讨SP在SOM中的作用。结果发现，在SOM条件下，中耳粘膜SP含量有逐渐增多趋势，并和MEE量呈正相关。每d腹腔注射1mg／mlspantide及1mg／miCimetidin均可使MEE明显减少，随着SOM时间的延长，两者使MEE减少的量并不一致，spantide减少MEE量更明显。提示：①SP可能是SOM的重要炎性递质；②SP可能通过组胺发挥其扩张血管增加血管通透性的作用，并可能直接作用于粘膜腺体促进分泌。

This experiment aimed to explore the effects of substance P(SP) on secretory oti-tis media (SOM ). Both immunohistochemistry ABC-GDN and image pattern analysis techniquewere adopted to investigate the relation between SP content of SOM middle ear mucosa and middleear effusion,and observe the effect of SP receptor antagonist spantide and histamine H2 receptorblocker cimetidin on middle ear effusion. The findings showed:middle ear mucosa SP content tend-ed to increase, and had positive correlation with middle ear effusion, intra-abdominally injecting1mg/ml Spantide and 1mg/ml Cinetidin per day could have middle ear effusion decreased obvious-ly,but the quantities of reduction were more significant- The results suggest that SP plays a role inSOM, might accelerate vasodilation and increase the permeability of cupillary in middle ear mucosamediated by histamine.