摘要
目的:研究死亡相关蛋白激酶(DAPk)基因在原发性胃癌组织中的表达,探讨其在胃癌发生发展中可能的作用。方法:收集62例原发性胃癌及相对应的癌旁正常组织。应用RT-PCR检测DAPk mRNA表达,免疫组化方法检测DAPk蛋白表达。结果:RT-PCR方法显示DAPk mRNA表达水平在胃癌组织中明显低于癌旁正常组织(OD值为0.2863±0.2027比0.5736±0.1968,P<0.0001)。免疫组化分析显示,DAPk在所有癌旁正常组织中表达均为阳性,积分范围为2-12,平均8.61±2.89;而在胃癌组织中表达则明显减弱或缺失,积分范围为0-9,平均2.90±3.38。DAPk在癌旁正常组织中的表达水平显著高于胃癌组织(P<0.0001)。结论:在原发性胃癌组织中,DAPk mRNA和蛋白表达明显缺失或低下。
Objective： To investigate the expression of death-associated protein kinase（DAPk）, an apoptosis-related serine/threonine kinase gene, in tissue of primary gastric cancer （GC） at mRNA and protein levels. Methods： DAPk mRNA expression was determined by RT-PCR, and the protein expression was measured using immunohistochemical staining in 62 GC tissues and adjacent normal tissues. Results： DAPk mRNA in GC were reduced significantly than in adjacent normal tissues （0. 286±3 0. 202 7 versus 0. 573 6±0. 196 8, P〈0. 000 1）. Immunohistochemical staining revealed that expression of DAPk was significantly lower in GC than in matched normal tissues （2. 90±3.38 versus 8. 61±2.89, P〈0. 000 1）. Conclusion： Expression of DAPk was significantly decreased in gastric cancer at mRNA and protein levels.
出处
《武汉大学学报（医学版）》
CAS
2007年第6期715-718,共4页
Medical Journal of Wuhan University
基金
湖北省科技攻关项目(编号:2004AA301C91)
关键词
死亡相关蛋白激酶
基因表达
胃癌
Death-Associated Protein Kinase
Expression
Gastric Cancer
