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PPAR-γ激动剂抑制子宫内膜基质细胞RANTES转录和翻译 被引量：1

PPAR-gamma Inhibits Endometrial Stromal Cell Transcription and Translation of RANTES in Vitro
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摘要 目的:探索过氧化酶增殖剂激活受体(PPAR)激动剂在人子宫内膜基质细胞中对于RANTES转录与翻译的作用。方法:Western blot测定PPAR-γ在人子宫内膜基质细胞中的表达;酶联免疫法测定PPAR-γ激动剂对于RANTES蛋白合成的作用;报告基因测定PPAR-γ激动剂对于RANTES转录的作用。结果:①PPAR-γ在子宫内膜基质细胞中表达;②PPAR-γ激动剂罗格列酮显著抑制RANTES的转录65%(P<0.01);③罗格列酮使子宫内膜基质细胞RANTES分泌减少48%(P<0.01)。结论:①子宫内膜基质细胞表达PPAR-γ;②PPAR-γ激动剂抑制子宫内膜基质细胞ANTES的转录和翻译。 Objective： To investigate the effect of Peroxisome Proliferator-Activated Receptor （PPAR-γ） ligands upon transcription and translation of RANTES in human endometrial stromal cells. Methods： Expression of PPAR-γ in endometrial stromal cells was detected by Western blot. The effect of PPAR-γ promoter on production of RANTES by enzyme-linked immunosorbent assay and gene sequencing assay. Results： In cells transfected with RANTES promoter vector, the addition of PPAR-γ ligands inhibited promoter activity by 65% （P〈0. 01）. Stromal cells showed a 48% decrease in RANTES protein secretion when treated with a PPAR-γ ligand （P〈0. 01）. Conclusion： The use of PPAR-γ ligands to chemokine production and inflammation may be a promising therapy for endometriosis.
作者 夏琼 赵栋 黄利红
机构地区 武汉市普爱医院妇产科 复旦大学附属中山医院妇产科
出处 《武汉大学学报（医学版）》 CAS 2007年第6期719-721,共3页 Medical Journal of Wuhan University
关键词 子宫内膜异位症 RANTES 过氧化酶增殖剂激活受体 Endometriosis RANTES PPAR-γ
分类号 R711.71 [医药卫生—妇产科学]
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参考文献10

  • 1Xu H,Kwon KB,Koo BS,et al.Regulated upon activation,normal T cell expressed and secreted(RAN-TES)and monocyte chemotactic protein 1 in follicular fluid accumulate differentially in patients with and without endometriosis undergoing in vitro fertilization [J].Fertil Steril,2006,86(6):1616-1620.
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二级参考文献20

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共引文献35

同被引文献7

  • 1于晓艳,李才,苗春生,周桂华.糖基化终末产物对大鼠肾皮质过氧物酶体增殖体激活受体-γ mRNA表达的影响[J].吉林大学学报（医学版）,2007,33(1):95-97. 被引量：5
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二级引证文献5

武汉大学学报（医学版）
2007年 第6期

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