青春期多囊卵巢综合征动物模型的实验研究

Establishment of Rat Model of Adolescence Polycystic Ovarian Syndrome

目的:建立脱氢表雄酮(DHEA)诱导未成熟雌性大鼠多囊卵巢综合征(PCOS)模型,并探讨其意义。方法:用DHEA皮下注射23d龄SD雌性大鼠,观察大鼠阴道口开放日龄及开口后阴道脱落细胞形态学特征,20d后取出卵巢、肾上腺、子宫称重,并观察各器官大体解剖及光镜(HE染色)特征,检测空腹血糖(FAS),测定血清睾酮(T)、黄体生成激素(LH)、空腹胰岛素(FIN)、胰岛素样因子(IGF-1)的水平。结果:①造模组较对照组大鼠阴道口开放日龄明显提前;造模组大鼠阴道脱落细胞无周期变化;②造模组卵巢、子宫、肾上腺湿重均高于对照组;组织学造模组肾上腺皮质网状带明显增宽,子宫内膜增厚,卵巢呈多囊改变,黄体数目显著减少;③造模组FAS、FIN、T和HOMA-IR均明显高于对照组;而LH值稍高于对照组;④造模组IGF-1显著低于对照组。结论:DHEA诱导大鼠PCOS模型与青春期PCOS部分特点相似,存在肾上腺初现功能亢进的特点,可作为研究青春期PCOS理想的动物模型。

Objective： To set up the rat polycystic ovarian syndrome （PCOS） model that induced by dehydroeplandrosterone （DHEA） for the further aclolescence PCOS research. Methods： Ten female SD rats aged 23 days were injected with DHEA, and the time of the aditus vaginae opened was recorded and the characters of vaginae cell morphology were observed. Another ten rats were used as controls. After 20 days of observation, the ovary, adrenal and uterus were weighed and the morphologic changes were analyzed. Fasting blood glucose, testosterone （T）, luteinizing hormone （LH）, fasting insulin （FIN） and serum insulin growth factor-1（IGF-1） levels in rat blood were also measured. Results： （1） The days of aditus vaginae opened in experiment group was significantly advanced than in control group. （2） The weight of ovary, adrenal and uterus, the length of uterus in experiment group were more than in control group. （3） The levels of FAS, T, FIN and HOMA-IR in the experiment group were higher than in control group, while the levels of LH had no difference between the two groups. （4） IGF-1 in the experiment group were significantly lower than in control group. Conclusion： DHEA-induced rat PCOS models were similar to some features of adolescence PCOS, and the Arch-adrenal hyperfunction was observed. It could be an ideal PCOS animal model for the research of adolescence PCOS.