联合接种法在假丝酵母菌培养中的应用

Combination of three kinds of inoculation methods in Candida spp. culture

目的寻找一种在最短时间内能为假丝酵母菌的鉴定提供最多信息的简易培养方法。方法在同一个平板内用分区划线法、多点接种法及小培养对标本进行培养,分离假丝酵母菌。结果一个平板内同时使用三种方法接种培养。可从分区划线法、多点接种法观察到菌落的全貌(如颜色、质地、形态)、种类、数量及生长特性等。可用显微镜从小培养中看到假丝酵母菌的孢子和菌丝的结构。结论由于假丝酵母菌种类繁多导致假丝酵母菌临床感染表现不同,临床诊断和治疗在很大程度上依赖于实验室的检查结果,因此寻找快速有效的鉴定方法至关重要。一个平板内同时用三种方法接种标本进行培养,可大大提高假丝酵母菌的检出率及鉴定效率,并能判定分离出的假丝酵母菌是目的菌株还是污染菌。

Objective To find a simple culture method in Candida spp. identification and get more data in the shortest time. Methods Streaking inoculation , multipoint inoculation and microculture were performed in one Sabourand＇s agar to isolate Candida spp. Results Perform streaking inoculation, multipoint inoculation and microcuhure in one Sabourand＇s agar. From Streaking inoculation and multipoint inoculation, we can observe the gross appearance of the colonies as the colour, form and texture, the number and growth habit, etc. From microculture we can observe the construction of hyphae and spores by microscopic examination. Conclusions There are various Candida spp. , many species lead to different clinic appearances of Candida spp. infection. Clinic diagnosis and treatment depend on laboratory results. It is important to find a simple method in fungi identifications. It is a good method that performing streaking inoculation ,multipoint inoculation and microculture in one Sabourand＇s agar. It can increase Candida spp. detection rates,improve strain identifecation efficiency obviously and differentiate aim fungi and pollution fungi.