神经生长因子干预对大鼠糖尿病性勃起功能障碍的影响

Study on effect of NGF in the intervention of erectile dysfunction induced by diabetes mellitus

目的:通过使用神经生长因子(NGF)干预糖尿病性勃起功能障碍(ED)大鼠,检测海绵体内压(ICP)和阴茎海绵体组织中含神经型一氧化氮合酶(nNOS)神经纤维的变化,以探讨NGF干预糖尿病性ED的作用机制。方法:将成年雄性SD大鼠分为5组:A组为正常对照组(6只),B组为糖尿病性ED无干预组(15只),C组为糖尿病性ED单用NGF干预组(14只),D组为糖尿病性ED单用胰岛素干预组(12只),E组为糖尿病性ED联用NGF、胰岛素干预组(13只)(胰岛素通过颈部皮下注射给药,NGF通过腹腔内注射给药)。采用腹腔注射STZ法制作糖尿病模型,造模8周后从糖尿病大鼠中筛选出合并勃起功能障碍大鼠:B(n=8),C(n=8),D(n=6),E(n=6),经药物干预后8周所有大鼠测ICP,并取阴茎海绵体组织用免疫组化EnVision法观察nNOS神经纤维的变化。结果:与B组相比,C、D、E组ICP水平均显著升高(P<0.05),其阴茎组织中含nNOS神经纤维水平也显著升高(P<0.05)。结论:给予外源性NGF可能有助于糖尿病性ED局部神经病变减轻和勃起功能改善,NGF在糖尿病性ED的发病中可能具有重要作用。

Objective：To investigate the mechanism of action of nerve growth factor（NGF） in penis of rats with diabetic ED by detecting the variation of intracavernous presure（ICP） and Nitric oxide synthase（NOS） containing nerve in the corpus cavernosum of the rat model who use NGF to treat the diabetic ED. Methods：All of 60 male Sprague-Dawley rats were divided into 5 groups：A（n = 6） control group,B（n = 15） diabetic ED group,C（n = 14） NGF intervention group,D（n = 12） insulin intervention group,E（n = 13）NGF plus insulin intervention group（use the insulin by subcutaneous injection and NGF by intraperitoneal injection）. Use the STZ i.p injected randomly to establish the dibetic model. Then the rats with diabetic ED were picked up after 8 weeks：B（n = 8）,C（n = 8）. D（n = 6）,E（n = 6）. Through 8 weeks of intervention,ICP was measured and the content of neuronal nitric oxide synthase（nNOS） in the erectile tissue was also evaluated by immunohistochemistry Staining. Results：Compared with those in group B,ICP was up-modulate significantly in intervened groups which received NGF or/and insulin intervention and the changes in nNOS staining of those groups were the same（P 〈 0.05）. Conclusion：The ability of combination of NGF with its receptor may also be damaged. It is helpful to use exogenous NGF to ameliorate the neuropathy and improve the erection function of diabetic rats. The abnormal of NGF may play an important role in the generation of diabetic ED and its treatment.