摘要
目的:探讨缬沙坦对过氧化氢(H2O2)致心肌细胞过氧化损伤的保护作用。方法:分离培养SD乳鼠心肌细胞,建立心肌细胞过氧化损伤模型。用2～10μmol/L缬沙坦预处理后,测定各组心肌细胞存活率;用10μmol/L缬沙坦预处理后,检测各组心肌细胞培养介质中乳酸脱氢酶(LDH)、丙二醛(MDA)及超氧化物歧化酶(SOD)浓度。结果:在2～10μmol/L范围内,缬沙坦剂量依赖性地提高心肌细胞过氧化损伤的细胞存活率(P<0.05);10μmol/L缬沙坦预处理后,培养介质中LDH和MDA的生成减少(P<0.05),而SOD含量增高(P<0.05)。结论:缬沙坦可能通过稳定细胞膜、抗脂质过氧化反应及提高清除氧自由基,对心肌心肌细胞过氧化损伤起剂量依赖性保护作用。
Objective：To investigate the protective effects of Valsartan（Val） on cardiomyocytes injured by hydrogen dioxide（H2O2）in neonatal SD rat. Methods：The protective effects of various concentration of Val on the model of cultured cardiomyocytes injured by H2O2 in vitro were evaluated by detecting the cell viability. The activity of lactate dehydrogenase（LDH）,malondialdehyde（MDA）,superoxide dismutase（SOD） were also assayed. Results：Val could protect the myocardial cells from H2O2 injury in a dose-dependent manner（2～10 μmol/L）, and Val（10 μmol/L） preconditioning showed the greatest protective effect. Val preconditioning could significantly attenuate the increased LDH 1eve1 and antagonize the elevated MDA level and the reduced SOD activity（P 〈 0.05）. Conclusion：Val might be has the protective effects on cardiomyocytes injured by H2O2 through improving cell antioxidant ablility.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第9期948-951,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助(30470709)
