混合菌群诱变及诱变菌群对闪锌矿浸出的影响

Mutagenesis of mixed bacteria and influence on bioleaching of sphalerite with the mutagenized bacterial admixture

将采自不同地区的混合菌分别培养于以黄铜矿和黄铁矿为能源的9 K培养基中,并以该培养物作为出发菌群,用亚硝酸钠(NaNO2)、硫酸二乙酯(DES)、紫外线(UV)及其组合为诱变剂,对混合菌群进行诱变。诱变后,经过连续浸矿筛选,得到对闪锌矿浸矿效果最好的诱变菌群E。浸矿试验结果表明:在9 K和Leathen培养基中,用诱变菌群E浸出闪锌矿40 d后,Zn2+的浸出率分别比对照组提高68%和84%;离心收集Leathen培养基中的菌体和矿样浸渣,弃除液体,加入新的Leathen培养基,在30℃和200 r/min条件下,恒温摇床培养20 d后,诱变菌群E对闪锌矿中Zn2+的二次浸出率比对照组高92%。

The mixed bacteria selected from the sites of different acid mines were respectively enriched in 9K and leathen medium plus chalcopyrite or pyrite as energy substances, then mutagenized with sodium nitrite, diethyl sulfate, ultraviolet（UV） and their combinations respectively. Leaching results show the mutagenized group E （mutagenized bacterial admixture） has much higher ore-leaching rate. Zn^2＋ leached from sphalerite is 68% and 84% higher respectively in the 9K and Leathen media inoculated with mutagenized group E than that in the controlled media inoculated with original （no-mutagenized） mixed bacteria. The content of leached Zn^2＋ increases by 92% compared with that of controlled group.