短萼海桐抗结核实验研究

Experimental study on antituberculous activities of Pittosporum brevicalyx(Oliv.)Gagnep

目的通过实验了解短萼海桐的抗结核活性。方法采用体外最低抑菌浓度(MIC)测定,含药血清MIC测定及体内抗结核动物实验。结果体外MIC测定短萼海桐在0．25～0．5 mg／ml浓度间出现明显抑菌现象;短萼海桐含药血清在80μg／ml浓度开始出现明显抑菌现象;体内抗结核实验显示短萼海桐能显著延长结核病模型小鼠的生存时间,提高存活率。结论短萼海桐在体内外显示明显的抗结核作用,需进一步研究确定是否可开发成为新型的抗结核天然药物。

Objective To study the antituberculous activities of Pittosporum brevicalyx （Oliv.）Gagnep. Methods The MIC of Pittosporum brevicalyx（Oliv. ） Gagnep and the sera containing Pittosporum brevicalyx（ Oliv. ） Gagnep were determined. The antituberculous effect of Pittosporum brevicalyx（ Oliv. ） Gagnep was evaluated in mouse model. Results The MIC of Pittosporum brevicalyx（Oliv. ）Gagnep and the sera containing Pittosporum brevicalyx（ Oliv. ） Gagnep were 0.25 - 0.5mg/ml and 80 μg/ml, repectively. Pittosporum brevicalyx（ Oliv. ） Gagnep could prolong the survival time and improve livability in the tuberculous mouse model. Conclusion Pittosporum brevicalyx（Oliv. ）Gagnep exhibited antituberculous activities in vitro and in vivo, and might be developed as a new crude antituberculosis drug.