槲寄生凝集素对人结肠癌HT-29细胞增殖及凋亡的影响

Effect of mistletoe lectins on proliferation and apoptosis in HT-29 colon cancer cell

目的:研究槲寄生凝集素对人结肠癌HT-29细胞增殖和凋亡的影响。方法:采用MTT法观察槲寄生凝集素对体外培养的人结肠癌HT-29细胞增殖的影响,运用基因组DNA电泳观察凋亡特征性"梯状"条带及原位末端标记法检测槲寄生凝集素诱导HT-29细胞凋亡。结果:槲寄生凝集素对体外培养的人结肠癌HT-29细胞抑制作用表现出时间和剂量依赖性。基因组DNA琼脂糖凝胶电泳显示槲寄生凝集素作用于HT-29细胞后出现凋亡细胞特有的DNA阶梯状条带。原位末端标记法检测显示槲寄生凝集素可明显诱导HT-29细胞的凋亡。结论:槲寄生凝集素可抑制人结肠癌HT-29细胞生长并诱导其凋亡。

AIM：To study the effect of mistletoe lectins on cell proliferation and apoptosis in HT-29 human colon cancer cell.METHODS：The effect of mistletoe lectins on cell viability in HT-29 cell was evaluated by MTT assay.Cell apoptosis was observed by DNA ladder analysis and Tunel.RESULTS：Mistletoe lectins（eg.,1-4 mg/L） inhibited cell growth in a dose and time dependent way in HT-29 cells.HT-29 cells apoptosis can be induced when exposed to mistletoe lectins（eg.,1-4 mg/L）.CONCLUSION：Mistletoe lectins can inhibits proliferation and induces apoptosis in HT-29 cells in vitro.