IL-8及CXCR-1在反流性食管炎患者食管黏膜中的表达及意义

Expression of interleukin-8 and CXCR-1 in esophageal mucosa of patients with reflux esophagitis

目的研究IL-8及其受体在反流性食管炎(reflux esophagitis,RE)食管黏膜上的表达水平,探讨其在RE发病中的意义。方法选择59例RE患者和20例对照者。在研究对象食管上取一块黏膜组织,予以免疫组化法检测食管黏膜IL-8、CXCR-1的蛋白水平。结果所有的RE患者食管黏膜上均有IL-8蛋白的表达,且均明显高于对照组(P<0.05),表达水平随着食管炎严重程度的升高而增高(P<0.05)。同正常对照组比较,RE患者食管黏膜上CXCR-1蛋白表达水平减少,但差异无显著性(P>0.05),不同程度RE组间的CXCR-1的表达差异也无显著性(P>0.05)。结论RE患者的食管黏膜中IL-8是高表达,IL-8是RE一个敏感的炎症参数,CXCR-1可能只是食管黏膜的构成蛋白。

Objective To study the expression of interleukin-8 and CXCR-1 in esophageal mucosa of patients with reflux esophagitis. Methods Fifty-nine patients with RE were classified into 4 grades according to Los Angeles classification and 20 normal controls were enrolled in the study. Biopsy sample was taken from the esophagus 3crn above the gastroesophageal junction, and formalin-fixed for immunohistochemical evaluation. Results The IL-8 protein expression level was significantly higher in esophageal mucosa of patients with reflux esophagitis than that of the controls（P 〈 0.05）, which was correlated with the endoscopic grade of esophagitis （P〈0.05）. There was no significant difference in CXCR-1 protein expression among the different endoscopic grades of RE and control group（P 〉 0.05）. Conclusion IL-8 level in esophageal mucosa is a sensitive inflammatory parameter in patients with reflux esophagitis. CXCR-1 maybe only a role constitutional protein of esophageal mucosa.