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Wnt_2B基因在胃癌组织及相应癌旁组织中的表达 被引量：1

Expression of Wnt_2B Gene in Gastric Carcinoma Tissues and Para-carcinoma Tissues
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摘要 目的:探讨Wnt2B基因在胃癌组织及相应癌旁组织中的表达及其意义。方法:采用逆转录多聚酶链式反应(RT-PCR)技术检测胃癌组织及相应癌旁组织中Wnt2B基因mRNA的表达水平。结果:Wnt2B基因在37例人类胃癌组织及相应癌旁组织中均有表达,阳性率达100%;Wnt2B基因在胃癌组织中的表达水平明显高于相应癌旁组织,差异具有统计学意义(P<0.001)。结论:Wnt2B基因可能参与了胃癌的发生发展过程,在胃癌组织中表达上调可能有助于胃癌的早期诊断。 Objective：To inv estigate the expression of Wnt2B gene in gastric carcinoma tissues and the corresponding paracarcinoma tissues and explore the relationship between the Wnt2B gene and gastric carcinogenesis.Methods： Total RNA was extracted from 37 cases of human gastric carcinoma tissues and the corresponding para-carcinoma tissues, and the levels of Wnt~ gene mRNA expression were evaluated by Reverse transcription-polymerase chain reaction （RT-PCR）. Results： The expression of Wnt2B gene mRNA could be detected in all the cases of gastric carcinoma tissues,and the corresponding para-carcinoma tissues. The level of Wnt2B gene mRNA expression in gastric carcinoma tissues was significantly up-regulated （P〈0.001）. Conclusion： Wnt2B gene participates in the gastric carcinogenesis. The up-regulated expression of Wnt2B gene mRNA during gastric carcinoma tissues may be of benefit for its prognosis.
作者 王晓峰 薛梅 杨连生
机构地区 南京市红十字医院消化内科
出处 《交通医学》 2007年第5期501-502,505,共3页 Medical Journal of Communications
关键词 Wnt2B基因 胃癌 逆转录多聚酶链式反应 Wnt2B gene gastric carcinoma RT-PCR
分类号 R735 [医药卫生—肿瘤]
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参考文献8

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二级参考文献20

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交通医学
2007年 第5期

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