胶质瘤中VEGF表达与微血管密度的关系及其临床意义

Study on Expression of Vascular Endothelial Growth Factor(VEGF), Microvessel Density(MVD) and Their Relationship in Gliomas

背景与目的:VEGF在肿瘤血管生成中起重要作用。本研究旨在探讨VEGF在人脑胶质瘤中的表达与微血管密度的关系及其临床意义。方法:采用免疫组化二步法检测36例人脑胶质瘤组织和8例非肿瘤对照脑组织中VEGF蛋白表达,采用CD34抗体标记测定微血管密度(miarovessel density,MVD)和并测算水肿指数(edema index,EI)。结果:(1)VEGF蛋白主要分布在高分级胶质瘤组织瘤细胞的胞浆内,低分级胶质瘤呈低水平表达,非肿瘤对照脑组织中VEGF蛋白均表达为阴性。VEGF在人脑胶质瘤中的总表达率77.8%(28/36)。(2)高分级(Ⅲ、Ⅳ级)胶质瘤MVD(68.88±19.05个/HP)也相对于低分级(Ⅰ、Ⅱ级)胶质瘤MVD(37.82±12.37个/HP)高(t=5.116,P<0.01)。(3)高分级(Ⅲ、Ⅳ级)胶质瘤水肿程度相对低分级(Ⅰ、Ⅱ级)胶质瘤程度较重。高分级胶质瘤EI与低分级胶质瘤EI之间有显著性差异(t=3.852,P<0.01)。(4)VEGF表达阳性者MVD(53.35±15.71个/HP)显著高于VEGF表达阴性者(15.76±5.38个/HP)(P<0.01),VEGF表达阳性者EI(3.45±1.63)显著高于VEGF表达阴性者(1.35±0.41)(P<0.05)。结论:VEGF的表达水平对判断人脑胶质瘤的临床病理级别有重要的价值。VEGF表达和MVD、EI检测结果可作为人脑胶质瘤生物学行为的判断指标。

BACKGROUND＆OBJECTIVE ： To investigate the expression of vascular endothelial growth factor （VEGF）,microvessel density（MVD） and their relationship in Gliomas. METHODS： Protein expression of VEGF in 36 human gliomas and 8 normal brain tissues were examined with two-step immunohistochemistry staining. Microvessel density（MVD） was stained with anti-CD34 monoclonal antibodies. Edema index（EI） was used to evaluate the extent of Peritumoural vasogenic brain edema（PVBE）. RESULTS： （1）Immunohistochemical staining for VEGF demonstrated that VEGF was strongly positive in high-grade gliomas, weakly positive in low-grade gliomas and negative in normal brain tissues. The VEGF positive rate in human gliomas was 77.8%（28/36）. （2）MVD in the malignant gliomas was greater than that in low grade gliomas（t=5.116, P 〈0.01）. （3）EI in the higt-grade gliomas was greater than that in low-grade gliomas（t=3.85,P〈0.01）. CONCLUSIONS： VEGF expression level is correlated with MVD and the pathological grade of gliomas.