鼻咽癌患者与EBV感染者外周血B淋巴细胞及其活化状况分析

Roles of the B-lymphocytes in peripheral blood of nasopharyngeal carcinoma patients and people infected by Epstein Barr virus

目的探讨鼻咽癌发生发展与B淋巴细胞及其活化状态的关系。方法运用流式细胞仪检测94例EBV感染者(其中鼻咽癌患者54例、非鼻咽癌不同抗体滴度者40例)外周血淋巴细胞CD19+、CD19+/CD21+的表达,并与健康人比较。结果鼻咽癌患者外周血淋巴细胞CD19+、CD19+/CD21+表达显著下降,EBV感染的低滴度组和高滴度非鼻咽癌组随着抗体滴度的增高,CD19+、CD19+/CD21+表达呈上升趋势。结论感染EBV的非鼻咽癌患者外周血B淋巴细胞中CD19+、CD19+/CD21+表达增多,处于活化状态的B细胞增多;EBV阳性的鼻咽癌患者外周血B淋巴细胞中CD19+、CD19+/CD21+表达率显著下降,处于活化状态的B淋巴细胞减少,提示B淋巴细胞免疫状态低下可能与鼻咽癌发生有关。

Objective To investigate the roles of the B-lymphocytes of peripheral blood in nasopharyngeal carcinoma（NPC） patients and people with different antibody titer, Methods Flow cytometry was used to detect CD19^＋ and CD19^＋/CD21^＋ expressions on B- lymphocytes of peripheral blood of 94 people infected by Epstein Barr virus （including 54 nasopharyngeal carcinoma patients） and normal controls. Results The expressions of CD19^＋ and CD19^＋/CD21^＋ on B-lymphocytes of peripheral blood in nasopharyngeal carcinoma patients were decreased significantly, while in non nasopharyngeal carcinoma patients infected by Epstein Barr virus, the expression of CD19^＋ and CD19^＋/CD21^＋ increased correspondingly as the antibody titer increased. Conclusion In nasopharyngeal carcinoma patients, the expressions of CD19^＋ and CD19^＋/CD21^＋ on B-lymphocytes of peripheral blood decreased significantly and the activated B-lymphocytes decreased. In non nasopharyngeal carcinoma patients infected by Epstein Barr virus, the expressions of CD19^＋ and CD19^＋/CD21^＋ increased slightly and the activated B-lymphocytes increased gently. These observations indicate that there may be some relation between B-lymphocytes and nasopharyngeal carcinoma.