腹腔注射刀豆蛋白A诱导昆明小鼠肝纤维化模型的建立

Establishment of the hepatic fibrosis animal model in KM mice by peritoneal injection with concanavalin A

目的建立昆明小鼠肝纤维化模型。方法48只昆明小鼠随机分组。实验组40只,腹腔注射20mg/kg剂量的刀豆蛋白A(ConA),每周一次,共9次。对照组8只,每周一次腹腔注射PBS。所有小鼠在每次注射后24h采血测ALT、AST。实验组分别于第6、7、8、9次注射后1周各处死8只小鼠,余8只第9周起停止注射,第13周处死。对照组第9次注射后1周处死。所有小鼠处死后取肝脏计算肝脏指数,并作病理检查。结果实验组第8次注射后出现典型肝纤维化,停止刺激4周仍然有纤维化表现。结论反复腹腔注射ConA可以建立昆明小鼠肝纤维化模型。

Objective To estabhsh hepatic fibrosis animal model in KM mice. Methods Forty eight KM mice were randomly divided into 2 groups. In the experimental mice, ConA at a dose of 20 mg/kg was injected into abdominal cavity weekly for 9 weeks. Eight mice in control group were injected with PBS instead of ConA. The sera were collected from all mice at 24 h after each injection for detecting alanine aminotransferase （ALT） and aspartate aminotransferase （AST）. In the experimental group, 8 mice were killed in one week after 6th, 7th, 8th, and 9th injection, respectively. The other 8 mice were not injected at 9th week and were killed at 13th week. The 8 mice in control group were killed at 9th week. The hver indexes were measured and pathological changes of the hver were observed. Results In the experimental group, typical hepatic fibrosis appeared after the 8th injection and lasted for 4 weeks after stopping injection. Conclusion The KM mice model of hepatic fibrosis can be established by intraperitoneal injection of ConA repeatedly.