阿霉素肾病模型鼠肾组织木糖基转移酶和乙酰肝素酶基因的表达

Gene Expressions of Xylosyltransferase and Heparanase in Renal Tissue of Rat with Adriamycin Nephropathy and Relevance for Proteinuria

目的研究肾病模型鼠肾组织木糖基转移酶(xylosyltransferase,XT-Ⅰ)和乙酰肝素酶(heparanase,Hpa)基因表达水平并观察其与尿蛋白量的关系。方法将大鼠随机分为正常对照组、肾病模型7d组、14d组、21d组,采用逆转录-聚合酶链反应(RT-PCR)对4组大鼠肾组织中XT-Ⅰ、Hpa基因表达进行半定量研究,并观察其与尿蛋白量的关系。结果肾病模型7d组、14d组、21d组与正常对照组之间比较,XT-Ⅰ表达量增加(P<0.05),肾组织XT-Ⅰ表达水平增高与尿蛋白的增加呈正相关关系(r=0.760,P<0.05);肾病模型7d组、14d组、21d组与正常对照组之间比较,HPa表达量增加(P<0.05),各组肾组织HPa表达水平增高与尿蛋白的增加呈正相关关系(r=0.757,P<0.05)。结论Hpa在大鼠肾病模型蛋白尿的产生中起重要作用,同时,XT-Ⅰ的增加可能导致硫酸乙酰肝素(HS)的合成增加。

Objective To evaluate the roles of β-D-xylosyltransferase I （XT- I ） and β-D-endoglycosidase heparanase （Hpa） in renal tissue with the development of adriamycin （ADR） nephropathy and relevance for proteinuria. Methods 150-200 g Sprague-Dawley rats （n= 10 per group） were used to make the nephropathy model induced by tail intravenous injection of 6 mg/kg ADR. Rats were housed in metabolic cage for 24 hours so as to collect the urine prior to disease induced and at day 7, 14 and 21 following disease induction. By using the method of reverse transcriptase-PCR （RT-PCR）, XT- I and Hpa genes from renal tissue of these groups were assayed respectively. Results （1） Rats with nephropathy at day 7, 14 and 21 showed the marked up-regulated XT- I mRNA and the significant difference in contrast to the normal rats,and rats at day 21 showed the marked up-regulated XT-I mRNA and significant difference in contrast to nephropathy rats at day 7. There was a positive linear correlation between the expression level of XT- I mRNA from the renal tissue of nephropathy rats and the quantity of urinary protein （r=0. 760, P〈0.05）. （2）The nephropathy rats at day 7, 14 and 21 showed marked up-regulated Hpa mRNA and significant difference in contrast to the normal rats,the rats at day 14 and 21 showed marked up-regulated Hpa mRNA and significant difference in contrast to the rats at day 7. There was a positive linear correlation between the expression level of Hpa mRNA from renal tissue and quantity of rat urinary protein （r=0. 757,P〈0.05）. Conclusion The up-regulated expressions of XT- I and Hpa mRNA in rat renal tissue exist in Adriamycin nephropathy. Higher Hpa mRNA expression, more quantity of urinary protein;more quantity of urinary protein,higher XT- I mRNA expression.