HPLC法测定甲基苯丙胺与苯丙胺

Detecting Methamphetamine and Amphetamine with High Performance Liquid Chromatography

目的建立甲基苯丙胺（methamphetamine，MA）和苯丙胺（amphetamine，AMP）的反相高效液相色谱测定方法。方法采用C18柱，以甲醇-磷酸盐缓冲液为流动相，流速1．0mL／min，检测波长为215nm，同时收集190～360nm的紫外光谱图，并以此与保留时间作为定性依据。在定性的基础上，建立定量检测方法，并对方法进行评价研究。结果所建方法能良好分离MA和AMP，结合判断依据能准确定性；MA在1．4～270μg／mL浓度范围内线性关系良好，R^2=1，日内标准偏差（RSD）与日间RSD均〈2．4％，检出限为0．73μg／mL，平均加样回收率为102．5％；AMP在0．9-580μg／mL浓度范围内线性关系良好，R^2=0．9999，日内RSD与日间RSD均〈2．3%，检出限为0．52μg／mL，平均加样回收率为101．7％。结论此方法简便、快速、准确，适用于MA与AMP的检测。

] Objective To establish a method for detecting methamphetamine （MA） and amphetamine （AMP） with high performance liquid chromatography （HPLC）. Methods Both MA and AMP were isolated on a C18 column and methanol-phosphate buffer （0. 015 mol/L NaH2PO4） at a flow rate of 1.0 mL/min. The 190-360 nm ultraviolet spectrum was examined, with 215 nm as the detection wavelength. Results The MA and AMP were well isolated and determined. The MA determined by the HPLC had good linearity with the real value at the range from 1, 4 to 270μg/mL （R^2=1）, with an average recovery rate of 102.5%. The detectable Limit was 0.73μg/mL （S/N≥3）. The AMP determined by the HPLC had a good linearity with the real value at the range from 0.9 to 580μg/mL （R^2=0. 9999）, with an average recovery rate of 101.7%. The detectable limit was 0.52μg/mL （S/N≥13）. Both intra-day and inter-day precisions expressed by relative standard deviations of the MA and AMP were less than 2.4%. Conclusion This is a simple, rapid and accurate method for detecting methamphetamine and amphetamine.