柱前紫外衍生-高效液相色谱法测定全氟辛酸

Detecting Perfluorooctanoic Acid by Reversed Phase High Performance Liquid Chromatography with Pre-column Ultraviolet Derivatization

目的建立污水和尿液中全氟辛酸（PFOA）的柱前紫外衍生一高效液相色谱测定方法。方法样品中的PFOA与四丁基氢氧化铵形成的离子缔合物被甲基叔丁基醚萃取后，与ω-溴苯乙酮在乙腈中衍生成具有紫外吸收的物质，高效液相色谱-紫外可见检测器检测。结果方法检出限可达7．0μg／L；相对标准偏差（RSD）〈10％；样品的回收率在85．3％～116％之间。结论本法适用于测定水样及尿液中的全氟辛酸。

Objectives To establish a reversed phase high performance liquid chromatographic method with pre-column ultraviolet derivatization for the determination of perfluorooctanoic acids （PFOA） in sewage and urine. Methods PFOA in sewage and urine formed an ion-pair with tetrabutylammonium （TBA） and were extracted into MTBE. The PFOA then reacted with co-bromoacetophenone （ω-BAP） in acetonitrile and produced materials quantifiable by HPLC with UV detector. Results The detection limit of the proposed method was 7.0μg/L with a relative standard deviation of less than 10%. The recoveries for real samples ranged from 85. 3% to 116%. Conclusion The proposed method is suitable for the determination of PFOA in sewage and urine.