摘要
目的:建立注射用己酮可碱中己酮可可碱含量及有关物质的高效液相色谱测定法。方法:采用Hy- persil C_(18)(150×4.6mm,5μm)化学键合柱,以甲醇-水-冰醋酸(35:65:1)为流动相,检测波长为274nm。结果;己酮可可碱的线性范围为40～800ng,r=0.9995,检出限为1.2ng,定量限为4.0ng。结论:该方法简便准确,可用于注射用已酮可可碱产品的质量控制和生产过程监测。
To establis a HPLC method to determine pentoxifylline and related substances in pentoxifylline for injection. Methods ： A Hypersil-C18 chemical-bonded column （150mm × 4.6mm, 5μm） was used with methanol-water-acetic acid （35：65：1） as mobile phase and detection at 274nm. Results： There was a good linear relationship in the range of 40-800ng （ r = 0. 9995 ） with detection limit of 1.2ng. Conclusion. The method is simple and accurate. It can be used for quality supervision and monitoring production process.
出处
《分析仪器》
CAS
2007年第4期54-57,共4页
Analytical Instrumentation
