鳜胸腺组织学结构及抗体分泌细胞的免疫组织化学

Histology structure of thymus and immunohistochemistry of antibody secreting cell in mandarin fish(Siniperca chuatsi)

使用常规组织学和免疫组织化学方法对鳜胸腺进行研究。观察到鳜胸腺位于鳃盖与背的联合处,呈三角形,对称分布;胸腺实质随年龄增长分叶逐渐明显,大体可分三个区,外区、中区和内区,中区和内区分别相当于哺乳动物的皮质区和髓质区。外区主要由扁平上皮细胞和粘液细胞组成,中区可观察到大量网状纤维和淋巴细胞,内区主要由排列较疏松的淋巴细胞组成。此外,光镜下还观察到哈氏小体和髓质上皮囊的存在。免疫组织化学结果显示胸腺中抗体分泌细胞主要分布在外区和中区,胸腺分叶部位尤为明显。阳性细胞胞膜着色明显,呈清晰棕黄色。

The thymus of mandarin fish （Siniperca chuatsi ）was studied by general histology method and immunohistochemical method. The thymus is located on the edge of the gill cover close to the opercular cavity, triangle,is distributed symmetrically. Lobules of thymus are more evident along with increasing age. The parenchyma of thymus is divided into outer zone, middle zone and inner zone, with middle zone and inner zone being equal to cortex and medulla of mammal thymus respectively. The outer zone chiefly consists of squamous epithelium cells and mucous cells. Large numbers of reticulate fibres and lympoid cells are shown in middle zone, and the inner zone is full of deep-blue staining lympoid cells which are distributed loosely. Furthermore, Hassal＇ s corpuscules and epithelial cysts are observed under light microscope. The result of immunohistochemistry shows that B lympoid cells of thymus are mostly distribute in the outer zone and middle zone , especially between the two lobules, cell membrane is positive evidently, brownish yellow.