摘要
对北极海冰获得的14株低温微生物进行培养条件和胞外水解酶活力的研究,结果表明:(1)菌株最适生长温度均为15℃和20℃左右,最适pH在8.0左右,在酸性条件下几乎不生长。生长需要一定浓度的盐,在3%NaC l的培养基中能很好的生长,而在没有NaC l的培养基中不生长。(2)14株菌株能不同程度的分泌胞外水解酶。(3)菌株i429,i539在低温下产纤维素酶的活力最高,具有低温酶的特性。
Studies was done on the culture condition and sreened of extracellurar hydrolase from 14 different cold adapted microorganisms. The result showed that optimal temperature of most of microorganisms was 15 ℃ and 20 ℃, The optimal pH at 8.0, hardly grown in acid condition, growth need the presence of NaCl, 14 strains produce extracellurar hydrolase with different activities, Strain i539 and i429 produced protease, cellulose and lipase, further results indicated that celluloses from i539 and i429 were low-tempature cellulose.
出处
《上海水产大学学报》
CSCD
北大核心
2007年第6期531-536,共6页
Journal of Shanghai Fisheries University
基金
国家自然科学基金(40376001)
关键词
低温微生物
筛选
蛋白酶
脂肪酶
淀粉酶
纤维素酶
cold adapted microorganism
screening
protease
lipase
amylase
cellulose