北极海冰低温微生物的生长条件及其胞外水解酶的研究

The studies of culture condition and extracellurar hydrolase of psychrophilic bacteria from Arctic sea ice

对北极海冰获得的14株低温微生物进行培养条件和胞外水解酶活力的研究,结果表明:(1)菌株最适生长温度均为15℃和20℃左右,最适pH在8.0左右,在酸性条件下几乎不生长。生长需要一定浓度的盐,在3%NaC l的培养基中能很好的生长,而在没有NaC l的培养基中不生长。(2)14株菌株能不同程度的分泌胞外水解酶。(3)菌株i429,i539在低温下产纤维素酶的活力最高,具有低温酶的特性。

Studies was done on the culture condition and sreened of extracellurar hydrolase from 14 different cold adapted microorganisms. The result showed that optimal temperature of most of microorganisms was 15 ℃ and 20 ℃, The optimal pH at 8.0, hardly grown in acid condition, growth need the presence of NaCl, 14 strains produce extracellurar hydrolase with different activities, Strain i539 and i429 produced protease, cellulose and lipase, further results indicated that celluloses from i539 and i429 were low-tempature cellulose.