摘要
目的了解门脉高压鼠血清一氧化氮（ＮＯ）含量变化及意义．方法以部分门静脉结扎大鼠为模型（ｎ＝１２），在部分门静脉结扎及假手术组大鼠（ｎ＝８）术后２周取门静脉血和周围静脉血，采用荧光分析法测量ＮＯ－２含量反应ＮＯ水平．结果门脉高压组门静脉血ＮＯ－２为０７６６μｍｏｌ／Ｌ±００９７μｍｏｌ／Ｌ，周围静脉血为０６８７μｍｏｌ／Ｌ±００９２μｍｏｌ／Ｌ，两者比较相差显著（Ｐ＜００１）；对照组门脉血ＮＯ－２为０６１３μｍｏｌ／Ｌ±００８４μｍｏｌ／Ｌ，周围血为０５９１μｍｏｌ／Ｌ±００４５μｍｏｌ／Ｌ，二者无明显区别（Ｐ＞００５）．门脉高压组与对照组比较，门脉血和外周血中ＮＯ－２含量均显著高于对照组（Ｐ＜００１）．结论门脉高压大鼠血清ＮＯ－２浓度升高，尤以门静脉血含量升高显著，表明门脉高压大鼠血中ＮＯ生成增多，可能在门脉高压症发病中具有一定作用．
AIM To evaluate the serum levels of NO and its function in the pathogenesis of portal hypertensive rats. METHODS The models of portal hypertension were established in Sprague Dawley rats ( n =12) by means of grade constriction of the portal vein. The concentrations of NO 2 - in the portal vein blood and peripheral blood of portal hypertensive and contol rats ( n =8) were measured with flourimetric analysis. RESULTS Serum concentrations of NO 2 - in the portal vein blood 0 766μmol/L±0 09μmol/L and peripheral blood 0 687μmol/L±0 092μmol/L were elevated obviously in the portal hypertensive rats as compared with those in the controls 0 613μmol/L,±0 084μmol/L, and 0 591μmol/L±0 045μmol/L respectively ( P <0 01). The levels of NO 2 - in the portal vein blood were markedly higher than those in the peripheral blood ( P <0 01) in the portal hypertensive rats. CONCLUSION Serum levels of NO 2 -, especially in the portal vein blood, are elevated in portal hypertensive rats and NO overproduction might play an important role in the pathogenesis of portal hypertension.
