摘要
目的：建立反相高效液相色谱法测定人血浆中甲氨蝶呤浓度的方法。方法：使用岛津LC-10AD高效液相色谱仪，LichrosphereC18柱（5μm，4.6mm×250mm），流动相为0.025mol·L^-1NaH2P04溶液（pH5.5）-甲醇（72：28），检测波长313nm，流速1.0mL·min^-1，柱温35℃，内标为阿魏酸。结果：甲氨蝶呤浓度在0．05～5．00μg·mL^-1范围内线性关系良好，回归方程为Y=3.0942c＋0．0068（r=0．9993），日内变异系数小于7％，日间变异系数小于10％；检测限为20ng·mL^-1。结论：本法操作简便，适用于甲氨蝶呤的血药浓度测定及药代动力学研究。
Objective：To establish a RP-HPLC method for the determination of methotrexate in human plasma. Methods： The LC-10AD HPLC apparatus and Lichrosphere C18 column （5 μm,4. 6 mmx250 mm） were used with a mobile phase consists of 0. 025 mol·L^-1 NaH2PO4 （ pH5.5 ） - methanol （72：28 ）. The detection wave- length was 313 nm. The flow rate was 1.0 mL·min^-1 Internal standard was ferulaic acid. Results： The linearrange of the calibration curve was 0. 05 - 5.00 μg·mL^-1 Linear equation ： r=3. 0942c ＋ 0.0068, r = 0.9993）. The inter-and intra-day precision were below 7% and below 10% respectively. The limit of detection was 20 ng·mL^-1. Conclusion ： The mothod is simple, which can be used in determination of methotrexate in human plas- ma and pharmacokinetic studies.
出处
《药学与临床研究》
2007年第5期390-392,共3页
Pharmaceutical and Clinical Research
基金
江苏省卫生厅科技发展项目(编号:P200404)
