0#柴油水溶性成分暴露对雌性褐菖鲉血浆卵黄蛋白原水平的影响

Effect of Water Soluble Fraction of 0# Diesel Oil on Vitellogenin Level in Serum of Female Sebastiscus marmoratus

雌性褐菖鲉通过水体分别暴露于0.02、0.10、.5 mg/kg的0#柴油水溶性成分,84 d后血浆睾酮水平与对照组相比显著下降;17β-雌二醇水平和血浆卵黄蛋白原水平下降;Western bolt检测显示柴油水溶性成分暴露组肝脏雌激素受体水平降低.雌二醇和雌激素受体水平降低是血浆卵黄蛋白原水平下降的主要原因.卵黄蛋白原水平下降将影响卵的发育和质量.

Female Sebastiscus marmoratus were exposed through the water to water soluble fraction of 0 # diesel oil （DWSF） at 0. 02,0.1,0.5 mg/kg, respectively. Serum testosterone levels decreased significantly, and serum 17β-estradiol and vitellogenin levels, which were analyzed by ELISA,decreased in the fish after exposure for 84 days compared with the corresponding controls. Estrogen receptor levels in the liver at the groups of DWSF exposure decreased compared with the corresponding controls analyzed by Western bolting. The decrease of 17β-estradiol and estrogen receptor levels would be principally causes of the decrease of vitellogenin, which would affect the development and quality of the ovum.